The supermarket giant has converted its store in High Holborn to checkout-free through using a new technology called GetGo.
Following the likes of Amazon, who use a similar format in their three ‘just walk out’ grocery stores that opened in London - as well as Aldi, which launched a till-free shop last month.
Customers will need to scan a QR code using their mobile phones, which allows them to pick up the items they need and walk straight back out.
Due to a “combination of cameras and weight sensors” that would establish what a customer had picked up, they would be charged through the Tesco. com app as soon as they left the store.
There will be a separate exit for those wishing to purchase age-restricted products where staff will check ID.
Staffing at the High Holburn store will not be affected, with Tesco confirming that they will continue to employ 22 workers in-line with other convenience stores.
Are there going to be any checkout-free Tesco stores in Newcastle?
Kevin Tindall, the managing director of Tesco Convenience, said: "We are constantly looking for ways to improve the shopping experience and our latest innovation offers a seamless checkout for customers on the go, helping them to save a bit more time.
"This is currently just a one-store trial, but we’re looking forward to seeing how our customers respond."